5000 u / ml Store at -20 ° C
CC ↑ TCGAGG
GGAGCT ↓ CC
Source: Arthrobacter species 7M06
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 µg / ml BSA; 7 mM 2-mercaptoethanol; 0.05% Triton X-100; 50% glycerol.
1X SEBuffer Abs I.
Incubate at 37 ° C.
The warranty period for storage of the enzyme at -20˚C is two years from the date of the last test indicated on the enzyme vial.
1X SEBuffer Abs I (pH 9.0 at 25ºC)
10 mM Tris-HCl, 50 mM KCl, 10 mM MgCl2, 1 mM DTT
Definition of unit:
One unit is defined as the amount of enzyme required to digest 1 µg of pUC19SE / DriI in 1 hour at 37 ° C in a total reaction volume of 50 µl.
Quality control tests
After a 2-fold over digestion with Abs I, ~ 90% of the DNA fragments can be ligated with T4 DNA ligase and re-cut.
A 50 µl reaction containing 1 µg of pUC19SE / DriI and 2 units of enzyme incubated for 16 hours resulted in the same DNA banding pattern as a reaction incubated for 1 hour. A long incubation time can cause star activity.
No detectable degradation of a single or double-stranded oligonucleotide was observed after incubation with 1 unit of enzyme for 3 hours.