Contamination in the laboratory is always a concern. We never know at what stage we see our culture marred by pollution. Many people use antibiotics in culture media to get rid of contaminants; Do you know that antibiotics not only negatively affect the growth and development of your crops but also do not ensure your cultivation with 100% efficacy? Some antibiotic-resistant microbes reappear and pose serious threats to your crops.
The solution to this major contamination problem is a Vegetable Preservative Blend (PPM). It is a trademark product of Plant Cell Technology that is now used in plant tissue culture laboratories around the world. PPM is the primary choice due to the extensive benefits it offers to cultivated plants. In this article, we will learn five main benefits of using PPM in your daily tissue culture routines.
Contamination in tissue culture occurs from different sources and different types of attack or contaminating cultures. It is difficult to find a solution for all these pollution problems. However, this problem can be easily addressed with PPM, a robust, broad-spectrum formulation for removing contamination from plant tissue culture. PPM protects the crop from all types of contamination: airborne, waterborne, or human-induced. Targets bacteria and fungi in plant tissue culture media and explanted tissue. PPM is an effective fighter against endophytic bacteria or microbes that live within plant tissues.
Some of the main applications of tissue culture are gene conservation, germination of haploid plants, and cultivation of hybrid plants. The only way that growers use to avoid contamination is the use of antibiotics. The result of antibiotics also alters the plant’s gene pool. If maintaining the genetic makeup of the plant is your primary requirement, then the use of antibiotics is clearly not a solution. So what should I do? What is the other way?
The other solution to the problem mentioned above is the use of PPM in your growing medium. PPM only helps protect your crop from contaminants and does not alter the genetic makeup of the plant!
What is your biggest concern during plant tissue culture? The perfect growth and development of plants and their good health throughout the different phases of tissue culture, right? But this goal is greatly affected by pollution. Here, we will talk about the impact of antibiotics and PPM to address this challenge.
Growers, when using antibiotics in their growing media, have observed that antibiotics protect the plant to some extent and at the same time negatively affect plant growth. In some plant species, it has been observed to lead to abnormal callus or aberrant tissue formation. In this case, PPM is preferred whose sole purpose is to fight with different classes of contaminants. It does not alter the genetic makeup of the plant or affect the growth of callus or explants in tissue culture.
Tissue culture is an expensive process. Although PPM is expensive, the value is really in the amount of PPM that is used to obtain a positive result. PPM is an effective solution to contamination problems with little or no limitation (if used in an adequate amount). Generally, 1-2 ml of PPM is required in 1 liter of culture medium and one liter of culture medium if you use 25 ml of medium per culture vessel. Then you can prepare 40 culture vessels. PPM is available at a reasonable price in plant cell technology in different volumes. You can check the PCT store for more information.
PPM is added to the culture medium before sterilization or after sterilization before dispensing into culture vessels. The best feature of PPM is that it can be autoclaved at 1.05 kg / cm2 (15 psi) and 121 ° C for 20 minutes. But, PPM must be added post-autoclaving to protein-containing media, before dispensing into culture vessels.
So now you know the top five benefits of using a plant preservative blend in your tissue culture routine. You can check our PCT store for more information on PPM or write to us if there is anything you want to know about PPM or any other plant cell technology product.